Biocatalysis as Useful Tool in Asymmetric Synthesis: An Assessment of Recently Granted Patents (2014–2019)

The broad interdisciplinary nature of biocatalysis fosters innovation, as different technical fields are interconnected and synergized. A way to depict that innovation is by conducting a survey on patent activities. This paper analyses the intellectual property activities of the last five years (2014–2019) with a specific focus on biocatalysis applied to asymmetric synthesis. Furthermore, to reflect the inventive and innovative steps, only patents that were granted during that period are considered. Patent searches using several keywords (e.g., enzyme names) have been conducted by using several patent engine servers (e.g., Espacenet, SciFinder, Google Patents), with focus on granted patents during the period 2014–2019. Around 200 granted patents have been identified, covering all enzyme types. The inventive pattern focuses on the protection of novel protein sequences, as well as on new substrates. In some other cases, combined processes, multi-step enzymatic reactions, as well as process conditions are the innovative basis. Both industries and academic groups are active in patenting. As a conclusion of this survey, we can assert that biocatalysis is increasingly recognized as a useful tool for asymmetric synthesis and being considered as an innovative option to build IP and protect synthetic routes

Contact values of the particle-particle and wall-particle correlation functions in a hard-sphere polydisperse fluid

The contact values $g(\sigma,\sigma')$ of the radial distribution functions of a fluid of (additive) hard spheres with a given size distribution $f(\sigma)$ are considered. A ``universality'' assumption is introduced, according to which, at a given packing fraction $\eta$, $g(\sigma,\sigma')=G(z(\sigma,\sigma'))$, where $G$ is a common function independent of the number of components (either finite or infinite) and $z(\sigma,\sigma')=[2 \sigma \sigma'/(\sigma+\sigma')]\mu_2/\mu_3$ is a dimensionless parameter, $\mu_n$ being the $n$-th moment of the diameter distribution. A cubic form proposal for the $z$-dependence of $G$ is made and known exact consistency conditions for the point particle and equal size limits, as well as between two different routes to compute the pressure of the system in the presence of a hard wall, are used to express $G(z)$ in terms of the radial distribution at contact of the one-component system. For polydisperse systems we compare the contact values of the wall-particle correlation function and the compressibility factor with those obtained from recent Monte Carlo simulations.Comment: 9 pages, 7 figure

Measurement of consensus with a reference

In this work we contribute to the formal analysis of the measurement of consensus in a society. Instead of approaching the topic from an absolute perspective we are concerned with a practical application: the proposal of a decision mechanism with respect to which consensus is measured. Surprisingly this produces a powerful unifying model, a restriction of which is deeply analysed. We also study the axiomatic properties of particular expressions for consensus with various salient social rules as a reference.Consensus; coherence; measurement; Borda rule; Copeland rule

Recent developments in the synthesis of β-diketones

Apart from being one of the most important intermediates in chemical synthesis, broadly used in the formation of C–C bonds among other processes, the β-dicarbonyl structure is present in a huge number of biologically and pharmaceutically active compounds. In fact, mainly derived from the well-known antioxidant capability associated with the corresponding enol tautomer, β-diketones are valuable compounds in the treatment of many pathological disorders, such as cardiovascular and liver diseases, hypertension, obesity, diabetes, neurological disorders, inflammation, skin dis-eases, fibrosis, or arthritis; therefore, the synthesis of these structures is an area of overwhelming interest for organic chemists. This paper is devoted to the advances achieved in the last ten years for the preparation of 1,3-diketones, using different chemical (Claisen, hydration of alkynones, decar-boxylative coupling) or catalytic (biocatalysis, organocatalytic, metal-based catalysis) methodologies: Additionally, the preparation of branched β-dicarbonyl compounds by means of α-functionalization of non-substituted 1,3-diketones are also discussed

Multienzymatic processes involving baeyer–villiger monooxygenases

Baeyer–Villiger monooxygenases (BVMOs) are flavin-dependent oxidative enzymes capable of catalyzing the insertion of an oxygen atom between a carbonylic Csp2 and the Csp3 at the alpha position, therefore transforming linear and cyclic ketones into esters and lactones. These enzymes are dependent on nicotinamides (NAD(P)H) for the flavin reduction and subsequent reaction with molecular oxygen. BVMOs can be included in cascade reactions, coupled to other redox enzymes, such as alcohol dehydrogenases (ADHs) or ene-reductases (EREDs), so that the direct conversion of alcohols or α,β-unsaturated carbonylic compounds to the corresponding esters can be achieved. In the present review, the different synthetic methodologies that have been performed by employing multienzymatic strategies with BVMOs combining whole cells or isolated enzymes, through sequential or parallel methods, are described, with the aim of highlighting the advantages of performing multienzymatic systems, and show the recent advances for overcoming the drawbacks of using BVMOs in these techniques.Ministerio de Ciencia e Innovación PID2019-105337RB-C22Banco Santander-UCM PR87/19-2267

The problem of collective identity in a fuzzy environment

Producción CientíficaWe define the problem of group identication in a fuzzy environment. We concentrate on the case where the society is required to self-determine the belongingness of each member to a speci_c group, characterized by a single attribute. In general terms, this case consists of a collective identity issue that can be regarded as an aggregation problem of individual assessments within a group. Here we introduce the possibility that both the original assessments and the resulting output attach partial memberships to the collectivity, for each potential member. We propose relevant classes of rules, and some are axiomatically characterized. Our new approach provides a way to circumvent classical impossibility results like Kasher and Rubinstein's.Ministerio de Economía, Industria y Competitividad (Project ECO2012-32178

Analysis of genetic markers in a sample of uruguayan criollo horses

The aim of this work is to charaterize at least tentatively the Criollo Horses of Uruguay through blood genetic markers. This breed, whose origin goes back to horses brought to Rio de la Plata in 1538 from Spain, nowadays, after more than four centuries in its environment, owns unique zootechnical traits and well developed rusticity. We have studied 7 systems of blood groups (A, C, D, K, P, Q and U) and 6 of biochemical polymorphisms (A1B, Al, Tf, alk-Es, PGD, PGM) in a sample of 99 Criollo Horses of Uruguay. The most polymorphic loci were the D blood group system with 13 alleles, Ddelo, Dadl and Ddkl being the 3 most frequent ones, and the Tf locus, that displayed 7 alleles, D and F2 having the highest frequency. The genetic variability of the sample was estimated through the average heterozigosity that reached a value of 0.435. Concerning the origin of the breed, we have to stress the presence in our sample of TfJ and Dcfgk, breed allele markers of the Spanish Pure Breed Horse. We conclude from these results that the present days Criollo Horses of Uruguay have preserved some blood genetic traits of its ancestors that differentiate them from the other Criollo Horses, displaying at the same time an important amount of genetic variability as shown by the high value of average heterozigosity.En esta comunicación preliminar, presentamos la evaluación de las frecuencias alélicas obtenidas en 7 sistemas de grupos sanguíneos (A, C, D, K, P, Q y U) y 6 polimorfismos bioquímicos (A1B, Al, Es alcalina, Tf, PGD y PGM) en una muestra de 99 Caballos Criollos del Uruguay (CCU). Los sistemas más polimórficos fueron el sistema D, con 13 alelos siendo los más frecuentes: Ddelo, Dadl y Ddkl, y el locus Tf con 7 alelos siendo los más frecuentes: D y F2. Todos los sistemas electroforéticos se encontraron en equilibrio génico. La variabilidad genética de la muestra estudiada se estimó mediante el Índice de Heterocigosidad esperada, el cual presentó un valor de 0,435. En la muestra analizada se destaca la presencia de la variante TfJ y del alelo Dcfgk, marcadores característicos de la Pura Raza Española (PRE). Se puede concluir que estos datos proporcionan información de que los Caballos Criollos Uruguayos conservan parte del potencial genético de sus ancestros, manteniendo características propias y diferentes a la de las otros Caballos Criollos. En cuanto a la variabilidad genética, a pesar de ser una muestra pequeña, presentó un índice de heterocigosidad bastante alto

Pairwise dichotomous cohesiveness measures

Producción CientíficaAbstract In a framework where experts or agents express their opinions in a dichotomous way, we analyze the cohesiveness of their opinions on axed set of issues in a population. A parametric family of related measures are introduced and axiomatically characterized. They are ordinally equivalent when the population isxed, and some further properties are proved. In order to argue that this restricted dichotomous situation is nevertheless versatile, the paper ends with several empirical illustrations based on real forecasts (for the 2012 American presidential election) and elections (with real data from referenda in two countries and from elections in several scientic societies).Ministerio de Economía, Industria y Competitividad (Project ECO2012-31933)Ministerio de Economía, Industria y Competitividad (Project ECO2012-32178)Ministerio de Economía, Industria y Competitividad (Proyect CGL2008-06003-C03-03/CLI)Ministerio de Economía, Industria y Competitividad (Project CGL2011-29396-C03-02)

Efecto de la densidad de siembra sobre el rendimiento de la okra (Abelmoschus esculentus (L.) Moench), en el valle de Lerma, Salta, Argentina

La okra es una malvácea anual cultivada como hortaliza adaptada a climas tropicales y subtropicales. El manejo del cultivo es poco conocido en Salta y Argentina. El objetivo fue determinar la densidad de siembra más adecuada en el valle de Lerma evaluando su efecto sobre las componentes del rendimiento. Con un diseño de bloques completos al azar, con 12 réplicas, se compararon cuatro densidades de siembra de okra variedad Blue Sky que fueron 1, 2, 3 y 4 plantas por pozo, (T1, T2, T3 y T4), con filas a 70 cm y hoyos a 30 cm. Se sembró el 25/10/11 y la emergencia fue a los 21 días. Se cosecharon frutos inmaduros entre el 23/12/11 y el 28/03/12, con un tamaño entre 7-10 cm de largo. Se evaluó el nú- mero y peso total de frutos cosechados en todo el ciclo de producción y por fecha de cosecha. Para todas las variables analizadas, los menores valores promedio se observaron en T1, mientras que entre T2, T3 y T4, si bien se vio una tendencia en aumento, no existieron diferencias estadísticamente significativas. Por consiguiente y debido a la dificultad de conseguir la semilla en el país y por su alto costo, se recomienda sembrar dos semillas por pozo.Okra is an annual Malvaceae cultivated as a vegetable crop and adapted to tropical and subtropical climate. There is no much crop management information in Salta and Argentina. The goal of this research was to determine the best plant density in the Lerma valley, Salta, Argentina. Treatments were four okra plant populations, cultivar Blue Sky (1, 2, 3 and 4 plants per hill: T1, T2, T3 y T4) evaluating its effect on the yield components. Experimental design was a randomized complete block with 12 blocks. The experiment was sown on October 25, 2011 and seed emergency happen 21 days after sowing. Fruits were harvested at immature stage from 23/12/11 to 28/03/12, with a size length from 7 to 10 cm. The parameters measured were: number and total fruit weight harvested in the whole production cycle and for each harvest date. For all parameters analyzed, the lower average values were observed in T1, but there were not statiscally differences between T2, T3 and T4. Nevertheless, there was a growing trend, although not statistically significant. It is recommended to plant two seeds per well (8 plants·m-2), because off the seed costs and for the difficulty of getting them in the Argentina.Fil: Lozano, L.. Universidad Nacional de Salta. Facultad de Ciencias Naturales; ArgentinaFil: Tálamo, Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Bio y Geociencias del Noroeste Argentino; Argentina. Universidad Nacional de Salta; ArgentinaFil: Galarce, M.. Universidad Nacional de Salta. Facultad de Ciencias Naturales; ArgentinaFil: Ruiz de los Llanos, R.. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentin

A MegaCam Survey of Outer Halo Satellites. VI: The Spatially Resolved Star Formation History of the Carina Dwarf Spheroidal Galaxy

We present the spatially resolved star formation history (SFH) of the Carina dwarf spheroidal galaxy, obtained from deep, wide-field g,r imaging and a metallicity distribution from the literature. Our photometry covers $\sim2$ deg$^2$, reaching up to $\sim10$ times the half-light radius of Carina with a completeness higher than $50\%$ at $g\sim24.5$, more than one magnitude fainter than the oldest turnoff. This is the first time a combination of depth and coverage of this quality has been used to derive the SFH of Carina, enabling us to trace its different populations with unprecedented accuracy. We find that Carina's SFH consists of two episodes well separated by a star formation temporal gap. These episodes occurred at old ($>10$ Gyr) and intermediate ($2$-$8$ Gyr) ages. Our measurements show that the old episode comprises the majority of the population, accounting for $54\pm5\%$ of the stellar mass within $1.3$ times the King tidal radius, while the total stellar mass derived for Carina is $1.60\pm0.09\times 10^{6} M_{\rm{\odot}}$, and the stellar mass-to-light ratio $1.8\pm0.2$. The SFH derived is consistent with no recent star formation which hints that the observed blue plume is due to blue stragglers. We conclude that the SFH of Carina evolved independently of the tidal field of the Milky Way, since the frequency and duration of its star formation events do not correlate with its orbital parameters. This result is supported by the age/metallicity relation observed in Carina, and the gradients calculated indicating that outer regions are older and more metal poor.Comment: Accepted in ApJ (22 pages, 13 figures